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Statistical design of the effect of inoculum conditions on growth of hairy root cultures ofCatharanthus roseus

✍ Scribed by Rajiv Bhadra; Jacqueline V. Shanks

Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
420 KB
Volume
9
Category
Article
ISSN
0951-208X

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✦ Synopsis

The factors in the inoculum conditions that influence the growth rate of hairy roots of Cutharunthus roseus in liquid culture were investigated in a randomized two-level, threefactor statistical design. The three variables were the number of root tips, the length of root tips, and the initial volume of media. The experiments and analysis demonstrated the effect of inoculum conditions on hairy root growth, with the length of hairy root tips being the dominant variable without any clonal variability. The best growth rates (doubling times) were obtained with an inoculum of 5 hairy root tips, each 35-40 mm long in 50 mL media: 3.7 and 3.1 days for C. roseus hairy root clones LBE-6-l and LBE-4-2, respectively.

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