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Statistical analysis of log-dose response bioassay experiments with unequal dose ratios for the standard and unknown preparations

✍ Scribed by C. Philip Cox


Publisher
John Wiley and Sons
Year
1967
Tongue
English
Weight
436 KB
Volume
56
Category
Article
ISSN
0022-3549

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✦ Synopsis


Permanganate oxidation of the metabolite of MTX yields a simple pteridine with the properties of 2,4-diamino-7-hydroxy-6-pteridinecarboxylic acid.

  1. Heating of the latter compound in 2 N sodium hydroxide solution at 70" leads t o slow hydrolysis of the 4-amino group.

  2. The nonpteridine moiety of the metabolite of MTX is identical with the nonpteridine moiety of MTX.

5 .

Tlie structure 4-amino-4-deoxy-7-hydroxy-N1o-methylpteroylglutamic acid ( 7-hydroxy-MTX) is proposed for the MTX metabolite.

REFERENCES

(1) Johns, D.