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Starburst low-molecular weight polyethylenimine for efficient gene delivery

โœ Scribed by Yanjun Zhao; Rulei Yang; Dong Liu; Mingjing Sun; Lijun Zhou; Zheng Wang; Ying Wan

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
492 KB
Volume
100A
Category
Article
ISSN
1549-3296

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โœฆ Synopsis

Abstract

Lowโ€molecular weight polyethylenimine (LMW PEI) shows the advantage of lowโ€cytotoxicity, but has been inefficient in gene delivery as a consequence of the lowโ€charge density. A number of previous studies employed the approach of crosslinking to solve this problem. In this study, a starburst LMW PEI gene vector has been developed. It has a polyamidoamine (PAMAM) core conjugated with a shell composed of LWM PEI and polyethylene glycol (PEG), that is PAMAMโ€PEIโ€PEG. Plasmid DNA (pEGFPโ€N1) and human cervix epithelial carcinoma (HeLa) cells were used in the study. The results showed that the starburst LMW PEI could effectively condense DNA at N/P above 5. The polyplexes had a size of about 500 nm and a nearly neutral surface because of the PEG shielding effect. This novel gene vector is able to maintain the lowโ€cytotoxicity of LMW PEI, whereas its transfection efficiency was significantly improved. ยฉ 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.

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