Standardized measurement of major immunoglobulin class (IgG, IgA, and IgM) antibodies to β2glycoprotein I in patients with antiphospholipid syndrome
✍ Scribed by Stephen Lewis; Lynn B. Keil; Walter L. Binder; Vincent A. DeBari
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 79 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0887-8013
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✦ Synopsis
Patients with the antiphospholipid syndrome (APS) have autoantibodies directed against epitopes on β 2 glycoprotein I (β 2 GPI). We describe herein the performance characteristics of standardized enzyme-linked immunosorbent assays (ELISAs) for antiβ 2 GPI of the three major immunoglobulin classes: IgG, IgA, and IgM. All three assays generated highly linear standard curves (5 points, r ≥ 0.993 for each); precision was excellent both intra-assay and run-to-run, with coefficients of variation (CV) ranging from 2.3% to 6.6%. Values for IgG anti-β 2 GPI correlated strongly with those obtained by an earlier method (r = 0.80, P< 0.0001). A study group consisting of 203 healthy subjects was used to generate percentile-based reference intervals for all three classes of anti-β 2 GPI. APS subjects' anti-β 2 GPI were found to differ significantly (P <0.0001 for each) from those of the control population. All three assays correlated well with β 2 GPIdependent anticardiolipin antibody (aCL) measurements; IgG: r = 0.94 (P <0.0001), for IgA: r = 0.82 (P<0.001) and for IgM: r = 0.84 (P<0.0001). We suggest that these ELISAs may provide valuable standardized measurements of IgG, IgA, and IgM antiβ 2 GPI.