Standardization of Romanowsky Stains
β Scribed by Marshall, P. N. ;Bentley, S. A. ;Lewis, S. M.
- Book ID
- 114789224
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 2009
- Weight
- 352 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0036-553X
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β¦ Synopsis
A panel of 17 eminent haematologists has assessed the performance of 5 Romanowsky stains prepared from pure component dyes, comparing the suitability and acceptability of these stains for the preparation of routine blood and boneβmarrow films. It was found that the results obtained using the stain described by Marshall et al (1975) were comparable to those obtained using a modification of the stain described by Wittekind et al (1976). The performance of the 3 other stains was less acceptable. Variations in stain formulation have been correlated with stain performance.
π SIMILAR VOLUMES
Romanowsky staining of suspension-fixed lymphocytes and fibroblasts, deposited as monolayers on slides, involves an initial basic dyeing process followed by formation of a hydrophobic Azur B/Eosin Y complex at the more permeable and so faster staining cellular sites. This mechanism is shared with bl