Introduction: Thallous chloride (Tl-201) radiopharmaceutical is routinely used for heart imaging in nuclear medicine departments. The goal of this research was to study the radioanalytical quality control of Tl-201 chloride using high performance liquid chromatography) HPLC). The HPLC method data were compared with data obtained from electrophoreses method recommended in usp.
Experimental: Thallous chloride ( 201 Tl(I)Cl) was obtained from NRCAM and oxidized slightly to 201 Tl(III)Cl 3 by O 3 . A cation exchange column (Hamilton PRP-X800,150Γ4.1mm), acetate buffer (100mM, pH=4.5 and flow rate=1 ml/min) as eluent and radioactive flow scintillation analyzer were applied for measurement. Free 201 Tl(I) and 201 Tl(III) have same properties over cation exchange resins and they can't be separated. DTPA solution was added to 201 Tl(I) and 201 Tl(III) for masking of 201 Tl(III) and separation of it from 201 Tl(I). Effect of DTPA solution concentration and pH was evaluated.
Results and Discussion: The retention time values of both free 201 Tl(I) and 201 Tl(III) were 8.6 min. The retention time value of 201 Tl(III)-DTPA complex was 1.3 min. The DTPA concentrations (0.02, 0.05, 0.1 molar) and pH(4-7) didn't affect the measured data. Data obtained from this method had good compatibility with data obtained from electrophoresis method recommended in USP.
Conclusion:
This method is short and accurate and can be used for routinely radioanalytical quality control of thallous chloride radiopharmaceutical.