Standard operating procedure for metabonomics studies of blood serum and plasma samples using a 1H-NMR micro-flow probe
✍ Scribed by Dinesh K. Sukumaran; Erwin Garcia; Jia Hua; Walter Tabaczynski; Kunle Odunsi; Chris Andrews; Thomas Szyperski
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 221 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0749-1581
- DOI
- 10.1002/mrc.2469
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✦ Synopsis
Abstract
A standard operating procedure (SOP) is presented for high‐throughput metabonomics studies using a 600 MHz NMR spectrometer equipped with a micro‐flow probe that is connected to an auto‐sampler. The procedure is designed to minimize random and systematic variation of NMR data collection. In addition, a protocol is described to assess the quality of the data acquired by a given NMR spectroscopist to ensure that (i) all researchers involved in the NMR data acquisition of a metabonomics research program perform equally and (ii) operator‐associated variation of NMR data collection is statistically not relevant for the interpretation of results obtained from multivariate data analyses. Copyright © 2009 John Wiley & Sons, Ltd.