Standard curve for housekeeping and target genes: Specific criteria for selection of loading control in Northern blot analysis

Comparison of Glyceraldehyde-3-phosphate

Comparison of Glyceraldehyde-3-phosphate Dehydrogenase and 28S-Ribosomal RNA Gene Expression as RNA Loading Controls for Northern Blot Analysis of Cell Lines of Varying Malignant Potential

✍ P. Bhatia; W.R. Taylor; A.H. Greenberg; J.A. Wright 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 366 KB

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been a gene of choice in Northern blot analyses as an internal RNA loading control. We have investigated the expression of GAPDH and 28S-ribosomal RNA (28S rRNA) genes in mouse 10T1/2 cells and in a variety of tumorigenic and highly malignant meta