Quantitation of acidic capsular polysacc
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Keith R. Powell; J.Owen Hendley; Karen E. Pohl; Anna Freidberg; Wesley A. Volk
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Article
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1982
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Elsevier Science
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English
β 449 KB
Alcian blue is a cationic dye used to stain acidic polysaccharides, including bacterial capsules, and as a biochemical reagent to quantitate glycosaminoglycans and glycoproteins. Alcian blue capsular polysaccharide (CP) complex formation can be quantitated by measuring the absorption at 620 nm of Al