✦ LIBER ✦

Staining of glycoproteins in polyacrylamide and agarose gels with fluorescent lectins

✍ Scribed by M. Furlan; B.A. Perret; E.A. Beck

Book ID: 102984367
Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 742 KB
Volume: 96
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90574-8

No coin nor oath required. For personal study only.

✦ Synopsis

We describe a simple and sensitive method for staining of the carbohydrate moiety of glycoproteins in polyacrylamide or agarose electrophoretic gels. Gels are incubated in a solution of fluorescein-labeled concanavalin A. Following destaining with a neutral buffer, glycoproteins exhibit fluorescence under long-range ultraviolet light. Thus, the glucose/ mannose cont~ning @ and y-chains of human fibrinogen give fluorescent bands, whereas the carbohydrate-free u-chain does not react. Less than 100 ng of hexose bound to fibrinogen /3-or y-chains could be detected. The procedure is suitable for staining of other carbohydrate residues in glycoproteins, which can be recognised by specific agglutinins, as shown by binding of fluorescein-labeled lectins from Ricinus communis to galactose residues of fibrinogen.

📜 SIMILAR VOLUMES

Visible fluorescent detection of protein

Visible fluorescent detection of proteins in polyacrylamide gels without staining

✍ Carol L Ladner; Jing Yang; Raymond J Turner; Robert A Edwards 📂 Article 📅 2004 🏛 Elsevier Science 🌐 English ⚖ 288 KB

The staining of sciatic nerve glycoprote

The staining of sciatic nerve glycoproteins on polyacrylamide gels with concanavalin A-peroxidase

✍ John G. Wood; F.O. Sarinana 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 557 KB

Rapid fluorescent staining of histones i

Rapid fluorescent staining of histones in sodium dodecyl sulfate-polyacrylamide gels

✍ Joan-Ramon Daban; Anna M. Aragay 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 657 KB

The increase in the fluorescence intensity of 1-anilinonaphthalene-8-sulfonate (ANS) produced by core histones is higher than that produced by very lysine-rich histones (H1 and H5). In the presence of the anionic detergent sodium dodecyl sulfate (SDS) the enhancement of ANS fluorescence caused by th

Identification of human platelet glycopr

Identification of human platelet glycoproteins in SDS-polyacrylamide gels using 125I labelled lectins

✍ J.L. McGregor; K.J. Clemetson; E. James; T. Greenland; M. Dechavanne 📂 Article 📅 1979 🏛 Elsevier Science 🌐 English ⚖ 464 KB

Staining of Concanavalin A-reactive glyc

Staining of Concanavalin A-reactive glycoproteins on polyacrylamide gels with horseradish peroxidase — a critical evaluation

✍ Dr. Klaus-Joachim Schott; Volker Neuhoff; Birgit Nessel; Ulla Pötter; Joachim Sc 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 668 KB

Improved Method for Silver Staining of G

Improved Method for Silver Staining of Glycoproteins in Thin Sodium Dodecyl Sulfate Polyacrylamide Gels

✍ H.J. Moller; J.H. Poulsen 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 281 KB

A method for detection of glycoproteins in thin sodium dodecyl sulfate polyacrylamide gels was developed by a combination of (i) initial periodic acid oxidation/Alcian blue staining and (ii) subsequent staining with silver nitrate. The procedure allowed detection of as little as \(1.6 \mathrm{ng}\)