Staining of extracellular polymeric substances and cells in bioaggregates
โ Scribed by Ming-Yuan Chen; Duu-Jong Lee; Joo-Hwa Tay; Kuan-Yeow Show
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 540 KB
- Volume
- 75
- Category
- Article
- ISSN
- 1432-0614
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## Abstract **BACKGROUND:** Over the last few decades, the emphasis in studies on extracellular polymeric substances (EPS) has been on the extraction and quantitative analysis methods relating to activated sludge. However, little is currently known about the relationship between EPS and microbial a
Scanning electron microscopy revealed that collapsed extracellular polymeric substances (EPS) surrounded bacteria present in granular sludge. Treatment of granular sludge with whole-cell antiserum and staining with polycationic ferritin demonstrated that bacteria were enveloped by extensive EPS. Ant