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Staining for enzymatic activity after gel electrophoresis: II. Enzymes modifying nucleic acids

✍ Scribed by Douglas M. Gersten; Othmar Gabriel


Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
707 KB
Volume
203
Category
Article
ISSN
0003-2697

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A Method for Activity Staining after Nat
✍ Jean Rivoal; Christopher R. Smith; Trevor F. Moraes; David H. Turpin; William C. πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 129 KB

We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m