✦ LIBER ✦

Staining for electron microscopy by vanadyl sulphate. Use of collagen as a model system

✍ Scribed by Margaret Tzaphlidou

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 447 KB
Volume: 19
Category: Article
ISSN: 0739-6260
DOI: 10.1016/0739-6260(88)90023-8

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✦ Synopsis

The staining behaviour ofvanadyl sulphate was studied using reconstituted collagen fibrils as a model system and comparing electron-optical data and collagen sequence data by a computer-aided correlation procedure. The results show that, under the conditions used, vanadyl sulphate stains both negatively and positively charged side-chains on the collagen. Other evidence suggests that vanadyl sulphate is an effective electron stain.

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