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Stable transformation of maize after gene transfer by electroporation

✍ Scribed by Fromm, Michael E.; Taylor, Loverine P.; Walbot, Virginia


Book ID
109742818
Publisher
Nature Publishing Group
Year
1986
Tongue
English
Weight
461 KB
Volume
319
Category
Article
ISSN
0028-0836

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Stable transformation via electroporatio
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Maize Type II callus tissue was used as the plant material for genetic transformation via electroporation. Plasmid DNA containing a selectable marker gene (either neomycin phosphotransferase (npt-II) or phosphinothricin acetyl transferase (bar)), and a screenable marker gene (gus A) was incubated wi