Stable transformation of barley callus using biolistic®particle bombardment and the phosphinothricin acetyltransferase (bar) gene
✍ Scribed by Carol M. Stiff; Andrzej Kilian; Huaping Zhou; David A. Kudrna; Andris Kleinhofs
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 735 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
✦ Synopsis
Suspension culture cells of barley (Hordeum vulgare L. cv. Klages) were collected on filters and bombarded with gold particles carrying the Basta ® resistance (bar) gene, or both bar and the 15-glucuronidase (uidA) genes in a Biolistic ® Particle Accelerator. Filters carrying the bombarded tissues were selected on basal medium with increasing concentrations (maximum of 200 mg 1-i) of phosphinothricin. Surviving calli were assayed for the presence of phosphinothricinacetyltransferase activity. All positivecalli were shown to have the bargene integrated into their genome. Nine independent stable transformation events have been analyzed with substantial variation in the integration pattern and transgene copy number. Some albino ptantlets were produced, but no mature plants were obtained.