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Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) for Studying Dynamics of Protein Abundance and Posttranslational Modifications

✍ Scribed by Amanchy, R.; Kalume, D. E.; Pandey, A.


Book ID
118127444
Publisher
American Association for the Advancement of Science (AAAS)
Year
2005
Tongue
English
Weight
696 KB
Volume
2005
Category
Article
ISSN
1945-0877

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## Abstract Analysis of protein complexes is of increasing interest in the field of proteomics. A challenge is to develop methods for monitoring changes in the quantity and subunit composition of protein complexes on a proteome‐wide scale. Here, we describe the combination of 1‐D blue native polyac