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Stable expression of human cytochrome P450IA2 cDNA in a human lymphoblastoid cell line: Role of the enzyme in the metabolic activation of aflatoxin B1

✍ Scribed by Charles L. Crespi; Dorothy T. Steimel; Toshifumi Aoyama; Harry V. Gelboin; Frank J. Gonzalez

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
367 KB
Volume
3
Category
Article
ISSN
0899-1987

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✦ Synopsis

Abstract

A human lymphoblastoid cell line stably expressing a human cytochrome P4501A2 cDNA was developed. This
recombinant cell line displayed P4501A2 protein and estradiol2‐hydroxylase activity, neither of which was detected
in the parental cell line. The recombinant cell line was also approximately 1000‐fold more sensitive t o the cyto‐
toxicity and mutagenicity of the carcinogenic mycotoxin aflatoxin B1 than was the parent cell line. The increase
in mutagenicity was supported by a corresponding increase in the level of aflatoxin B1 binding t o DNA in cells
expressing P4501A2 relative to control cells.

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