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Stabilization, partial purification and characterization of peptidyl peptide hydrolases from germinated barley

✍ Scribed by W.C. Burger; Neville Prentice; Mary Moeller; G.S. Robbins


Book ID
108364832
Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
702 KB
Volume
9
Category
Article
ISSN
0031-9422

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Purification, stabilization and characte
✍ Shoji Ida; Eigo Mori; Yuhei Morita πŸ“‚ Article πŸ“… 1974 πŸ› Springer-Verlag 🌐 English βš– 712 KB

Nitrite reductase (NiR) isolated from barley (Hordeum vulgare L.) roots was stabilized in a buffer solution containing a sulfhydryl-reducing reagent and glycerol. The enzyme was purified 340fold by ammonium sulfate fractionation and chromatography on DEAE-Sephadex A-50, Sephadex G-200 and DEAE-cellu