✦ LIBER ✦
Stabilization of λ repressor against thermal denaturation by site-directed Gly→Ala changes in α-helix 3
✍ Scribed by Michael H. Hecht; Julian M. Sturtevant; Robert T. Sauer
- Book ID
- 105358360
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 332 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0887-3585
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✦ Synopsis
Oligonucleotide-directed mutagenesis has been used to replace alpha-helical glycines in the N-terminal domain of lambda repressor with alanines. Since alanine is a significantly better helix-forming residue than glycine, these changes were predicted to have a stabilizing effect. We show that the Gly46----Ala substitution, the Gly48----Ala substitution, and the double substitution increase the melting temperature of the N-terminal domain by 3-6 degrees.