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Stabilization of pyrroloquinoline quinone glucose dehydrogenase by cross-linking chemical modification

✍ Scribed by Koji Sode; Tomonori Shimakita; Shokichi Ohuchi; Tomohiko Yamazaki

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
285 KB
Volume
18
Category
Article
ISSN
0141-5492

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✦ Synopsis

Cross-linking chemical modification of pyrroloquinoline quinone (PQQ) glucose dehydrogenase (GDH) by glutaraldehyde was carried out and its stability was analyzed. Although native PQQGDH was inactivated within 30 min at a higher temperature than 50 cross-linked PQQGDH retained more than 40% of initial activity even after 30 min of incubation at 54Β°C. In addition to the increase in thermal stability, cross-linked PQQGDH gained high EDTA tolerance. The stabilization may be achieved by increased the rigidity of PQQGDH holo enzyme conformation.

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