Experiments in homogenous solution confirm previous findings that catalase is an effective oxidant for Hgo+ Hg2+. Peroxidase also effects the oxidation, and in both oxidations the co-substrate hydrogen peroxide is not essential for mercury oxidation, although the oxidation is enhanced by the presenc
Stabilization of peroxidase-antibody conjugate in solution by polyethylene oxide
β Scribed by Scott Boyd; Hiroshi Yamazaki
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 329 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0951-208X
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β¦ Synopsis
The inclusion of polyethylene oxide to a peroxidase-antibody conjugate suspension greatly stabilized conjugate activity which showed no loss during storage for 36 days at 30Β°C. This stabilization not only economizes enzyme inununoassays but also permits its use under field conditions lacking refrigeration.
π SIMILAR VOLUMES
The purpose of this study was to develop a novel synthetic stabilizer of enzyme-linked antibody in the enzyme-linked immunosorbent assay (ELISA). The watersoluble amphiphilic phospholipid polymer, poly[2methacryloyloxyethyl phosphorylcholine (MPC)-co-styrene (St)] was synthesized, and its stabilizin