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Stabilization of pepsin on duolite for the continuous hydrolysis of bovine haemoglobin at pH2 and 40°C

✍ Scribed by F. Sannier; J. M. Piot; D. Guillochon; P. Dhulster; D Thomas

Book ID: 104643656
Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 360 KB
Volume: 7
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00151085

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✦ Synopsis

Several methods of pepsin immobilization have been applied in order to achieve the continuous hydrolysis of a 2,5% haemoglobin solution at pH 2 and 40°C. Methods using glutaraldehyde were unsuccesful because of the unstability of the derived enzyme at low pH. Pepsin covalently bounded to a Duolite amine resin by a carbodiimide showed a half life of 15 days during the hydrolysis of haemoglobin in a column reactor, No enzyme activity was detected in the hydrolysates. No accumulation of haem in the column was noticed which could have limited long term studies by plugging the system. Modulation of the degree of hydrolysis was also performed by changing the feeding flow rate of the reactor.

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