It is well known that excipients are required to protect nonviral vectors during the lyophilization process. The goal of this study is to describe the stability of lyophilized nonviral vector preparations on pharmaceutically relevant timescales and provide insight into the factors that govern long-t
Stabilization of lipid/DNA complexes during the freezing step of the lyophilization process: the particle isolation hypothesis
โ Scribed by S.Dean Allison; Marion d.C Molina; Thomas J Anchordoquy
- Book ID
- 117047667
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 310 KB
- Volume
- 1468
- Category
- Article
- ISSN
- 0005-2736
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๐ SIMILAR VOLUMES
The incorporation of components with covalently attached polyethylene glycol (PEG) into nonviral vectors has been shown to prevent aggregation in serum and extend the circulating half-life of lipid/DNA complexes (lipoplexes) in vivo. The tendency of synthetic vectors to aggregate during processing a
The instability of nonviral vectors as liquid formulations has stimulated considerable interest in developing dehydrated formulations that would be resistant to shipping stresses and could be stored at room temperature. Recently, we reported that high sucrose/DNA ratios are capable of maintaining pa
The stresses during the secondary-drying stage of lyophilization were investigated using a controlled humidity mini-freeze-dryer [Luthra S, Obert J-P, Kalonia DS, Pikal MJ. 2007. Investigation of drying stresses on proteins during lyophilization: Differentiation between primary and secondary-drying