Stabilization of ferrocene leakage by physical retention in a cellulose acetate membrane. The fructose biosensor

✍ Scribed by Ján Tkáč; Igor Voštiar; Peter Gemeiner; Ernest Šturdı́k

• Book ID: 104443879
• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 96 KB
• Volume: 55
• Category: Article
• ISSN: 1567-5394
• DOI: 10.1016/s1567-5394(01)00130-x

✦ Synopsis

The prevention of ferrocene leakage from an electrode by physical retention of mediator in a matrix of cellulose acetate membrane is reported. Five types of the cellulose acetate membranes were prepared, containing 1.8%, 5.3%, 8.5%, 20.0% of ferrocene and a membrane containing 1.8% of ferrocene and 0.05 % of NafionR in the matrix. Ferrocene embedded membranes were successfully applied in the construction of a fructose biosensor by immobilization of PQQ-dependent fructose dehydrogenase (FDH). The biosensor comprising a cellulose acetate membrane with 1.8% of ferrocene and 0.05% of NafionR had good stability characteristics, retained almost 40% of the initial response after 8 h of continuous use with an initial sensitivity of 226 nA mM À 1 and response time of 75 s.