Stabilization of double-stranded DNA molecule by nonhistone peptidic effector from calf thymus

✍ Scribed by G. L. Gianfranceschi; D. Amici; L. Guglielmi

• Book ID: 104738409
• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 483 KB
• Volume: 3
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00357209

✦ Synopsis

A peptidic effector from calf thymus causes a strong stabilization of DNA double-stranded molecule in vitro. The active factor was isolated from aqueous ultrafiltered thymus extracts and purified by means of chromatography on DEAE-cellulose and then on Dowex 50 WX2. The purified thymic factor was characterized as a peptide of low molecular weight (less than 5000). The biological activity of the thymic factor cannot be attributed to a histone fragment. Melting data of the control DNA and of the DNA-active factor complex in various conditions of ionic strength and dielectric constant of the solution medium are recorded.