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Stabilization of antibody VH-domains by proteolytic selection

โœ Scribed by Hiroshi Ueda; Peter Kristensen; Greg Winter


Book ID
113803767
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
264 KB
Volume
28
Category
Article
ISSN
1381-1177

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Secondary antigen stimulation usually produces IgG antibodies with hypermutated V segments. Studying a strong secondary response to the polynucleotide antigen poly(dC), however, we found a highly selective IgG antibody (mAb dC7) with only one mutation (a conservative Leu to Ileu substitution) throug