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Stabilization of a Transition-State Analogue at the Active Site of Yeast Cytosine Deaminase: Importance of Proton Transfers

✍ Scribed by Xu, Qin; Guo, Haobo; Gorin, Andrey; Guo, Hong


Book ID
121325535
Publisher
American Chemical Society
Year
2007
Tongue
English
Weight
290 KB
Volume
111
Category
Article
ISSN
0022-3654

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Six fully conserved arginine residues (R129, R131, R235, R291, R319, and R340) closely grouped in the nucleotide binding site of rabbit muscle creatine kinase (rmCK) were mutated; four to alanine and all six to lysine. Kinetic analyses in the direction of phosphocreatine formation showed that all fo