Stability studies of the components of a prototype penicillinase (β-lactamase)-linked ELISA kit

✍ Scribed by M. I. Khatkhatay; M. P. Desai; G. M. Sankolli; D. K. Pardhe; U. M. Joshi

• Book ID: 102307789
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 428 KB
• Volume: 7
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860070205

✦ Synopsis

Penicillinase (p-lactamase) enzyme-linked immunosorbent assay (ELISA) for various reproductive hormones developed in the laboratory were found to have wide applicability in the fertility check clinic of the Institute. A need was thought to transform these assays into ready-to-use kit forms. Therefore, prototype ELISA kits for these hormones were developed and stability of the individual component was ascertained at various temperatures (room temperature, 37°C and 2-8°C).

Stability studies were conducted on previously validated assay for pregnanediol-3a-glucuronide (PdG). The studies showed that immunosorbents (antibody coated plates) are stable at room temperature for a period of 2 weeks, at 37°C for 1 week and at 2 4 % for a period of 9 months when preserved after treatment with glycerol solution. The lyophilised conjugate, standard and immunoassay buffer, colour reagent, and its substrate were stable at 37°C up to 1 week and at room temperature up to 2 weeks and at 2-8°C for a period of 6 months, during which the stability was studied. o 1993wiley-Liss, Inc.