The ability of two high-performance liquid chromatography gel permeation columns to separate proteins was evaluated. These columns gave satisfactory molecular weight separations for some, but not all, proteins tested. These results indicate that there are limitations in confidence of molecular weigh
Stability of serum γ-glutamyltranspeptidase fractionated by gel permeation using high-performance liquid chromatography and fast protein liquid chromatography
✍ Scribed by Seijin Hosaki; Junko Sawamoto; Michio Hagiwara; Takami Kusano; Hiroyuki Oda; Shin-Ichi Shiina
- Book ID
- 108449428
- Publisher
- Elsevier Science
- Year
- 1989
- Weight
- 188 KB
- Volume
- 488
- Category
- Article
- ISSN
- 0378-4347
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Cardiodilatin (CDD), a polypeptide exhibiting vasorelaxant and diuretic natriuretic bioactivity, was isolated from bovine atria. The isolation procedure reported here is different from that originally used for the purification of porcine and bovine CDD. Instead of cation-exchange chromatography on F
Some results and observations concerning the use of protein columns are presented. The combined use of four protein columns having different fractionation ranges together with a volatile triethylamine formate buffer allowed the sieving of various polypeptides according to their molecular weights ove