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Stability of immobilized α-chymotrypsin in supercritical carbon dioxide

✍ Scribed by Pedro Lozano; Antonio Avellaneda; Raphaël Pascual; José L. Iborra

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
393 KB
Volume
18
Category
Article
ISSN
0141-5492

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✦ Synopsis

Inactivation of immobilized c~-chymotrypsin in supercritical carbon dioxide was with a first-order kinetic behaviour. The increase in either the pressure or the temperature of the fluid enhanced the inactivation process of the enzyme. The fluid density was shown as a key parameter on the enzyme stability, enhancing the half-life time proportionally to the physical phase of CO2, as follows: liquid > supercritical > gas. However, the number of pressurization/depressurization cycles, and the water content of the derivative increased greatly the loss of activity.

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