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Stability of hepatitis C virus RNA in serum from samples collected in a closed-tube system for serum separation and transport, as measured by a quantitative competitive PCR assay

✍ Scribed by M-A. Trabaud; F. Bailly; G. Colucci; C. Trepo


Book ID
115076189
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
205 KB
Volume
3
Category
Article
ISSN
1352-0504

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A modified competitive RT-PCR (mcRT-PCR) to measure HCV RNA in serum and the Amplicor HCV Monitor assay were compared. For mcRT-PCR, the RNA extracted was retrotranscribed and coamplified in one step with a known amount of a DNA internal control (IC). Digoxigenin-labeled amplified products were hybr