✦ LIBER ✦

Stability of etiochloroplasts isolated from pine cotyledons as studied by means of low temperature absorption and fluorescence spectroscopy

✍ Scribed by M. Brouers; M. R. Wolwertz

Book ID: 104614615
Publisher: Springer
Year: 1980
Tongue: English
Weight: 523 KB
Volume: 1
Category: Article
ISSN: 0166-8595
DOI: 10.1007/bf00018226

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✦ Synopsis

77 K absorption and fluorescence spectra axe measured during incubation of etiochloroplast isolated from pine cotyledons and suspended in buffers with or without various co-factors (GSH, ATP, CoA*) and NADPH. It is shown that, in the absence of co-factors, rapid spectral changes due to denaturation of the protein-pigment complexes occur. The spectral changes differ according to whether denaturation occurs in the light or in the dark. The rate of denaturation of the Chl-protein complexes is significantly lowered when co-factors are added and a protective effect of NADPH on the PChlide-protein complexes was observed.

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