✦ LIBER ✦
Sporulation: An alternative way to recover recombinant proteins from Bacillus subtilis
✍ Scribed by Giovanni Maurizi; Paola Bossù; Diana Boraschi; Edy Ulisse; Aldo Tagliabue; Paolo Ruggiero
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 407 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
A recombinant strain of Bacillus subtilis engineered for endocellular expression of human interleukin-I receptor antagonist (IL-I ra) was subjected to sporulation. The recombinant protein was recovered from the sporulation supernatant in quantities, purity, and activity comparable with those obtained from a traditional cell lysate. Thus, exploitation of this natural mechanism of autolysis could overcome problems of intact protein recovery related to the cell disruption step.