✦ LIBER ✦

Spontaneous apoptosis of tumor-specific tetramer+ CD8+ T lymphocytes in the peripheral circulation of patients with head and neck cancer

✍ Scribed by Andreas E. Albers; Carsten Schaefer; Carmen Visus; William Gooding; Albert B. DeLeo; Theresa L. Whiteside

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 205 KB
Volume: 31
Category: Article
ISSN: 1043-3074
DOI: 10.1002/hed.21031

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Background

In cancer, tumor escape from the host immune system includes apoptosis of circulating CD3^+^CD8^+^ effector T lymphocytes. Here, we compare sensitivity to apoptosis of virus‐ with tumor‐specific circulating CD8^+^ T cells in patients with head and neck cancer.

Methods

Wild‐type p53 peptide‐specific (p53~264‐272~ and p53~149‐157~) and viral peptide‐specific (EBV BMLF~259‐267~ and CMVpp65~495‐503~) tetramers were used to measure the frequency of reactive T cells by flow cytometry. Annexin V (ANX) binding to circulating 7‐amino‐actinomycin D‐negative but tetramer^+^CD8^+^ T cells in PBMC obtained from 21 patients with head and neck cancer and 11 normal controls (NC) was evaluated.

Results

In patients with head and neck cancer, a higher percentage of tetramer^+^CD8^+^ than tetramer^−^CD8^+^ T cells bound ANX (p < .023–.005). Although most tumor‐epitope^+^CD8^+^ T cells bound ANX, lower percentages of virus‐specific CD8^+^ T cells were ANX^+^ in the same patients.

Conclusions

📜 SIMILAR VOLUMES

Tumor-specific CD8+ T lymphocytes derive

Tumor-specific CD8+ T lymphocytes derived from the peripheral blood of prostate cancer patients by in vitro stimulation with autologous tumor cell lines

✍ Franck Housseau; Daniel A. Langer; Samuel D. Oberholtzer; Anitha Moorthy; Hyam I 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 150 KB 👁 2 views

## Abstract To identify tumor‐associated antigens as putative targets for developing immunotherapies against prostate cancer, we investigated the ability of T cells derived from the peripheral blood lymphocytes of prostate cancer patients to recognize autologous tumor cells. The technical challenge

Differences in the recognition of tumor-

Differences in the recognition of tumor-specific CD8+ T cells derived from solid tumor, metastatic lymph nodes and ascites in patients with gastric cancer

✍ Koji Kono; Fumiko Ichihara; Hidehiko Iizuka; Takayoshi Sekikawa; and Yoshirou Ma 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 43 KB 👁 2 views

We established gastric cancer-specific CD8 1 T-cell (T CD8 1) lines derived from different lymphocyte sources in the same patients by repeated stimulation with mitomycin-C-treated autologous tumor cells with low-dose interleukin-2, and we compared recognition patterns among the T CD8 1 derived from

Relationships between regulatory T cells

Relationships between regulatory T cells and CD8+ effector populations in patients with squamous cell carcinoma of the head and neck

✍ Kazuaki Chikamatsu; Koichi Sakakura; Theresa L. Whiteside; Nobuhiko Furuya 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 323 KB 👁 1 views

## Abstract ## Background Homeostasis of circulating T cells is regulated in complex ways that have not yet been well defined. The balance between type 1 and type 2 T‐cell subsets in cancer patients is thought to modulate antitumor immunity. Meanwhile, CD4+CD25+ regulatory T cells (Treg), which ar