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Spontaneous activity and recurrent inhibition in cultured hippocampal networks

✍ Scribed by Dr. Mario Siebler; Hubertus Köller; Christine C. Stichel; Hans Werner Müller; Hans-Joachim Freund

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
765 KB
Volume
14
Category
Article
ISSN
0887-4476

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✦ Synopsis

Abstract

As a model for an integrated neuronal network based on the concept of modular units, we have investigated the occurrence of spontaneous activity and the formation of synaptic circuits in primary cultures of dissociated hippocampal neurons from the embryonic rat. Sodium‐dependent action potentials (APs) could be elicited after 1 day in vitro (DIV), whereas spontaneous postsynaptic potentials (PSPs), “miniature” PSPs and APs appeared after 3‐6 DIV. The number of cells with spontaneous APs and the rate of APs increased during development of the neuritic network. In addition to a stochastic spike interval distribution, pyramid‐shaped neurons could be identified after 10‐12 DIV, which fired preferentially at interspike intervals between 20‐120 ms and 190‐400 ms. This distinctive bimodal interspike interval pattern was sensitive to GABA‐A antagonists. Simultaneous recordings of pairs of neurons demonstrated recurrent inhibitory, GABA‐ergic synaptic circuits. In addition, a subpopulation of GABAergic neurons could be visualized by immunocytochemistry. These results are discussed in relation to the hypothesis that spontaneous firing of connected neurons is networkdriven, based on synaptic “noise” and patterned by recurrent inhibition. © 1993 Wiley‐Liss, Inc.

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