Spliced segments at the 5′ terminus of adenovirus 2 late mRNA. Susan M. Berget, Claire Moore and Phillip A. Sharp; An amazing sequence arrangement at the 5′ ends of adenovirus 2 messenger RNA. Louise T. Chow, Richard E. Gelinas, Thomas R. Broker and Richard T. Roberts
✍ Scribed by Reviewed by Tim J. Harrison
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 830 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1052-9276
No coin nor oath required. For personal study only.
✦ Synopsis
hexon) mRNA using R-looping with restriction fragments of the adenovirus 2 genome. Late in infection, most RNA on polysomes is viral and hexon mRNA is the most abundant species. Rlooping exploits the higher melting temperature of RNA : DNA than DNA : DNA duplexes, using electron microscopy to visualise structures in which hybridised RNA has displaced one of the DNA strands from a duplex molecule. Similar studies were carried out in Roberts' laboratory at CSHL. The results of these experiments were to revolutionise our understanding of eukaryotic transcription.
Spliced segments at the 5k terminus of adenovirus 2 late mRNA* (adenovirus 2 mRNA processing/5k tails on mRNAs/electron microscopy of mRNA . DNA hybrids)