𝔖 Bobbio Scriptorium
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Spleen autotransplantation in mice: A novel experimental model for immunology study

✍ Scribed by Irén Mikó; Endre Bráth; István Furka; Judit Kovács; David Kelvin; Robert Zhong

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
35 KB
Volume
21
Category
Article
ISSN
0738-1085

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The aim of the experimental model to develop a spleen transplant model in mice to study the role of spleen in autoimmune and transplant rejection. After a midline incision, splenectomy was performed. Four tiny segments were cut from the removed spleen and were rinsed at room temperature in physiological salt solution. The greater omentum was lifted and four omental pockets were created; four thin segments were then placed into the “nests,” subsequently marked, and fixed using 8‐0 suture. The abdomen was then closed. The duration of the survival time was different among the nine groups (n = 3–3). Tissue samples were taken from the marked areas for histological examination stained with hematoxylin and eosin (H&E). H&E staining demonstrated large, well‐circumscribed splenic nests with lymphoid zone and red pulp and well‐formed trabecules in the spleen. Among the possible applications of this novel model is the ability to study the role of spleen in autoimmune and organ rejection.

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