Spiro indolinone beta-lactams, inhibitors of poliovirus and rhinovlrus 3C-proteinases

✍ Scribed by Jerry W. Skiles; Daniel McNeil

• Publisher: Elsevier Science
• Year: 1990
• Tongue: French
• Weight: 250 KB
• Volume: 31
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(00)88543-3

✦ Synopsis

Preparation of the spiro beta-lactam 7 and its evaluation as a poliovirus and human rhmovirus 3Cproteinase inhibitor is described.

Controlled proteolysis of precursor polypeptides is crucial to the life cycle of picomavirusesl (e.g., human rhinovirus and poliovirus). The replication of many animal and plant viruses is entirely dependent on pmteolytic processing, and proteolytic enzymes are involved in a variety of functions including separation of structural and non-structural proteins, generation of specific enzymes (RNA polypeptides), coordinated assembly of the virion and maturation. The evidence suggests the involvement of virus-encoded proteinases in the processing of viral precursor polypeptides. The rhinovirus, like other picomaviruses contains a singk positive strand RNA genome that is translated into a large single precursor polyprotein 000 Daltons). This polyprotein can cleave itself into different domains without the help of cellular components. It does this so efficiently that under normal