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Spin Trapping of Nitric Oxide by Nitronylnitroxides: Measurement of the Activity of NO Synthase from Rat Cerebellum

✍ Scribed by Y.Y. Woldman; V.V. Khramtsov; I.A. Grigorev; I.A. Kiriljuk; D.I. Utepbergenov


Book ID
115574943
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
450 KB
Volume
202
Category
Article
ISSN
0006-291X

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A simple and sensitive assay method for NO synthase activity is described. Using glassy carbon as electrode and 30% methanol solution with 10mM NH&I as mobile phase, NO, can be measured without disturbing ECD-detectable substance in NO synthase assay mixture. The NO; production in the assay mixture