Spin Labeling: Theory and Applications

✍ Scribed by David J. Schneider, Jack H. Freed (auth.), Lawrence J. Berliner, Jacques Reuben (eds.)

• Publisher: Springer US
• Year: 1989
• Tongue: English
• Leaves: 661
• Series: Biological Magnetic Resonance 8
• Edition: 1
• Category: Library

✦ Synopsis

We present this special topics volume on an area which has not received thorough coverage for over 12 years. Spin Labeling: Theory and Applications represents a complete update on new theoretical aspects and applications of the spin-label method. In the "line-shape theory" sections, we are especially pleased to include an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations. Barney Bales discusses inhomogeneous broadening phenomena in detail. Several developments in techniques and interpretation in saturation transfer spectroscopy have appeared since the publica­ tion of Spin Labeling II: Theory and Applications (L. J. Berliner, ed., Academic Press, 1979). We have included an up-to-date chapter on spin-label applications by M. A. Hemminga and P. A. de Jager. By incorporating 15N and deuterium into nitroxide spin labels, several unique advantages are derived in line-shape analysis. Albert Beth and Bruce Robinson have contributed a detailed chapter on the analysis of these labels in the slow-motion regime while Jane Park and Wolfgang Trommer present the advantages for specific biochemical examples in our "applications" section. Derek Marsh's contri­ bution on spin-label spectral analysis may be regarded as a summary chapter which touches on several of the detailed spectral analysis methods described in the earlier chapters.

✦ Table of Contents

Front Matter....Pages i-xix
Calculating Slow Motional Magnetic Resonance Spectra....Pages 1-76
Inhomogeneously Broadened Spin-Label Spectra....Pages 77-130
Saturation Transfer Spectroscopy of Spin Labels....Pages 131-178
Nitrogen-15 and Deuterium Substituted Spin Labels for Studies of Very Slow Rotational Motion....Pages 179-253
Experimental Methods in Spin-Label Spectral Analysis....Pages 255-303
Electron-Electron Double Resonance....Pages 305-307
Resolved Electron-Electron Spin-Spin Splittings in EPR Spectra....Pages 339-397
Spin-Label Oximetry....Pages 399-425
Chemistry of Spin-Labeled Amino Acids and Peptides....Pages 427-488
Nitroxide Radical Adducts in Biology: Chemistry, Applications, and Pitfalls....Pages 489-546
Advantages of 15 N and Deuterium Spin Probes for Biomedical Electron Paramagnetic Resonance Investigations....Pages 547-595
Magnetic Resonance Study of the Combining Site Structure of a Monoclonal Anti-Spin-Label Antibody....Pages 597-613
Approaches to the Chemical Synthesis of 15 N and Deuterium Substituted Spin Labels....Pages 615-634
Back Matter....Pages 635-650

✦ Subjects

Biochemistry, general;Biotechnology;Analytical Chemistry;Biophysics and Biological Physics