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Spin-echo 1H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte

✍ Scribed by J. Reglinski; W. E. Smith; R. D. Sturrock


Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
378 KB
Volume
6
Category
Article
ISSN
0740-3194

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✦ Synopsis


Human erythrocytes were subjected to oxidative stress using arsenicals. The study is a model for thiol-related redox processes observed in the etiology of diseases such as rheumatoid arthritis. Spin-echo NMR spectroscopy of the living cell was used to monitor the cellular biochemistry. Oxidation of glutathione and the first demonstrated response of ergothioneine to a chemical stimulus in a cell were observed. The reversible nature of the ergothioneine response is interpreted as an environmental rather than a chemical change.