Spermine modulation of specific [3H]-gabapentin binding to the detergent-solubilized porcine cerebral cortex α2δ calcium channel subunit

Isolation of the [3H]Gabapentin-Binding

Isolation of the [3H]Gabapentin-Binding Protein/α2δ Ca2+Channel Subunit from Porcine Brain: Development of a Radioligand Binding Assay for α2δ Subunits Using [3H]Leucine

✍ Jason P. Brown; Visaka U.K. Dissanayake; Alex R. Briggs; Mihajlo R. Milic; Nicol 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 225 KB

The novel antiepileptic agent gabapentin (Neurontin) binds with high affinity to the alpha 2 delta subunit of a voltage-dependent Ca2+ channel. We report here a simple purification scheme for detergent-solubilized alpha 2 delta subunits from porcine brain. This involves sequential chromatography on