Spermidine and flower-bud differentiation in thin-layer explants of tobacco
โ Scribed by R. Kaur-Sawhney; A. F. Tiburcio; A. W. Galston
- Publisher
- Springer-Verlag
- Year
- 1988
- Tongue
- English
- Weight
- 522 KB
- Volume
- 173
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
โฆ Synopsis
Three lines of evidence indicate a connection between high spermidine levels and floral initiation in thin-layer tissue cultures of Wisconsin-38 tobacco (Nicotiana tabacum L.). (1) Spermidine levels are much higher in floral buds than in vegetative buds. (2) Inhibition of spermidine synthesis by cyclohexylamine prevents the rise in spermidine titer, inhibits floral initiation and promotes the formation of vegetative buds instead. (3) Application of exogenous spermidine causes floral initiation in cultures which would otherwise form vegetative buds.
๐ SIMILAR VOLUMES
Regeneration of flower buds in thin tissue layers from pedicels of photoinduced short-day (SD) tobacco, Nicotiana tabacum L. cv. Maryland Mammoth, is described. Up to seven flower buds per explant were obtained in a medium containing Murashige and Skoog's macro- and microclements, 100 mg/l myoinosit