Speeding Up Biomolecular NMR Spectroscopy
β Scribed by Bernd Simon; Michael Sattler
- Publisher
- John Wiley and Sons
- Year
- 2004
- Weight
- 51 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0931-7597
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β¦ Synopsis
Abstract
For Abstract see ChemInform Abstract in Full Text.
π SIMILAR VOLUMES
Nuclear magnetic resonance is an important tool for high-resolution structural studies of proteins. It demands high protein concentration and high purity; however, the expression of proteins at high levels often leads to protein aggregation and the protein purification step can correspond to a high
High-speed MAS gives a three-fold gain in resolution of the m++m -I ( m = 3/Z and -I /2) satellite transitions of I= 5/2 nuclei in polycrystalline samples compared with the conventional "central transition" spectroscopy. This is demonstrated using a standard high-field NMR spectrometer and samples o