Spectroscopic membrane probes. Volume 2

✍ Scribed by Loew, Leslie M

• Publisher: CRC Press
• Year: 2018
• Tongue: English
• Leaves: 217
• Series: CRC revivals
• Category: Library

✦ Synopsis

The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane. The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably, emerged directly from probe studies over the last 20 years. This work is designed to make these techniques accessible to a broad audience of cell biologist. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on required apparatus, experimental design, data analysis, and potential pitfalls. Read more...

Abstract: The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane. The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably, emerged directly from probe studies over the last 20 years. This work is designed to make these techniques accessible to a broad audience of cell biologist. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on required apparatus, experimental design, data analysis, and potential pitfalls

✦ Table of Contents

Content: Cover
Title Page
Copyright Page
DEDICATION
ACKNOWLEDGMENTS
PREFACE
THE EDITOR
CONTRIBUTORS
Table of Contents
Chapter 9: Fluorescence Correlation Spectroscopy and Photobleaching Recovery: Measurement of Transport and Chemical Kinetics
Chapter 10: Cell Surface Clustering and Mobility of the Liganded LDL Receptor Measured by Digital Video Fluorescence Microscopy
Chapter 11: Total Internal Reflection Fluorescence: Theory and Applications at Biosurfaces
Chapter 12: Near-Field Imaging of Fluorescence Chapter 13: Fluorescent Probes of Anion Transport Inhibition in the Human Red Cell MembraneChapter 14: How to Choose a Potentiometric Membrane Probe
Chapter 15: Potential-Sensitive Molecular Probes in Energy-Transducing Organelles
Index

✦ Subjects

Cell membranes.;Spectrum analysis -- Technique.;SCIENCE / Life Sciences / Anatomy & Physiology.