Spectroscopic investigation on the toxic interaction of melamine with herring sperm DNA

✍ Scribed by Yajing Sun; Rutao Liu; Zhenxing Chi; Pengfei Qin; Xiaoyan Fang; Yue Mou

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 180 KB
• Volume: 24
• Category: Article
• ISSN: 1095-6670
• DOI: 10.1002/jbt.20341

✦ Synopsis

Abstract

The toxic interaction of melamine with herring sperm DNA (hs‐DNA) was investigated by using fluorescence and UV–vis absorption spectra techniques. The experimental results showed that the toxic interaction between melamine and hs‐DNA occurred. Fluorescence quenching experiments indicated the existence of electrostatic binding between melamine and hs‐DNA. The binding constants K~A~ and the binding site numbers were calculated by means of the Stern–Volmer equation and were 9.8 × 10^4^ L mol^−1^ and 1.3, respectively. Both the results of fluorescence spectra and UV–vis absorption spectra verified that there are electrostatic binding between melamine and hs‐DNA. The possibility in the presence of a classical intercalation binding mode could be ruled out by using DNA unwinding experiments. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:323–329, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20341