Spectrophotometry of human hemoglobin in the midinfrared region
โ Scribed by J. Todd Kuenstner; Karl Norris; Victor F. Kalasinsky
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 147 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1075-4261
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โฆ Synopsis
Absorbance spectra for the hemoglobin species, including oxy-, deoxy-, carboxy-, and methemoglobin in the midinfrared region, are presented. The absorbance spectra of all species in aqueous solution are similar with absorption bands centered at approximately 3280, 3080,
๐ SIMILAR VOLUMES
Quantitation of small amounts (2-3 pg) of hemoglobin (Hb) was achieved by each of three spectrophotometric techniques. An assay employing benzidine base consistently underestimated Hb at this level and gave a wide scatter of results at higher values. The cyanmethemoglobin method was at the limit of
The opinions or assertions contained herein are the private views of the authors, and are not to be construed as official or reflecting the views of the Department of the Army or the Department of Defense.