Spectrophotometric titration studies on poly (uridylic acid)

✍ Scribed by H. Simpkins; E. G. Richards

• Book ID: 101714799
• Publisher: Wiley (John Wiley & Sons)
• Year: 1967
• Tongue: English
• Weight: 463 KB
• Volume: 5
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1967.360050609

✦ Synopsis

In order to provide preliminary data for the interpretation of the spectrophotometric titration properties of RNA, the spectral changes accompanying the ionization of poly-(uridylic acid) have been determined, as have the ionization constants as a function of salt concentration and the enthalpies and entropies of ionization. The spectral properties and ionization constants of poly(uridy1ic acid) have been compared with those of 2'(3')-uridine monophosphate and of uridine; significant differences have been established.

The results obtained are consistent with the hypothesis that uracil residnes in poly U are stacked only in concentrated salt solutions.