✦ LIBER ✦
Spectrophotometric, potentiometric, and density gradient ultracentrifugation studies of the binding of silver ion by DNA
✍ Scribed by Ronald H. Jensen; Norman Davidson
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1966
- Tongue
- English
- Weight
- 809 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
The equilibrium and the stoichiometry for the reversible complexing of silver ion by DNA have been studied by potentiometric titrations, proton release pH-stat titrations, and by spectrophotometry. The complexing reactions involve primarily the purine and pyrimidine residues, not the phosphate groups. There are at lea& three types of binding (types I, 11, and 111), of which the h t two have been intensively studied in this work. The sum of type I and type I1 binding saturates at one silver atom per two nucleotide residues. In the type I and type I1 reactions, zero and one proton, reapec-