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Spectrophotometric pH titrations and nitration with tetranitromethane of the tyrosyl residues in yeast phosphoglycerate kinase

✍ Scribed by Thomas Hjelmgren; Lars Arvidsson; Märtha Larsson-Raźnikiewicz


Book ID
115734148
Publisher
Elsevier Science
Year
1976
Weight
443 KB
Volume
445
Category
Article
ISSN
0005-2744

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Fully deuterated yeast phosphoglycerate kinase ([2H]PGK) was prepared biosynthetically with only histidine side chains of normal (1H) isotopic composition. The 1H NMR spectrum of this enzyme ([1H]His[2H]PGK) showed that the histidine side chains are clearly visible as sharp signals. Thus detailed st